Alternate header for print version


Reconstruction
Display image description
Maximum intensity projection through an optical section series/mosaic of a Purkinje neuron from the mouse cerebellar cortex injected with Lucifer Yellow and imaged with multiphoton microscopy. Both the original data and a deconvolved version are available for download. The deconvolved data can be viewed through the animation link for this data set.
Full resolution image description
Zip archive containing: 1) reconstructed mosaic optical sections in Tiff format (Mosaic Tiff Series folder) without deconvolution; 2) Deconvolved images along with the PSF used to perform the deconvolution (Deconvolution folder); Rotation loops of original and deconvolved data (Movie file). The movies are both full resolution (Full.avi) and downsampled (Fit.avi) for both original and deconvolved data. Total file size is 431 Mb.
Volume_dimension
1919, 2675, 109
Volume scale
0.118, 0.118, 0.5
Animation description
Rotation loop of a maximum intensity projection through a projection of an optical section series/mosaic of a Purkinje neuron filled with Lucifer Yellow and imaged with multiphoton microscopy. The image stack was deconvolved. The non-processed data can be viewed in the display image.

Image 2D
Display image description
Portion of a filled Purkinje cell injected with Lucifer Yellow and imaged using multiphoton microscopy as a high resolution mosaic. The entire cell is viewable in the reconstruction section. The original unprocessed mosaic and z stack is available for download.
Full resolution image description
Entire unprocessed multiphoton mosaic and z stack in a single file (pur_img.img) in RTS2000 custom .img image format. Please contact us if there is trouble reading this file and we will convert it into a different format.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3687*
Project: P1170
Project name
Mouse BIRN test data
Description
Neurolucida tracing of filled Purkinje neurons
Funding agency
NIH
Leader(s)
Maryann Martone
Collaborator(s)
Diana Price
Andrea Thor
Masako Terada
Hiro Hakozaki
Start date
03-01-2002
End date
unspecified
 
Experiment
Experiment ID
3433
Title
High resolution mosaic of Purkinje neuron
Purpose
To obtain a high resolution mosaic of a filled Purkinje neuron and test deconvolution software
Experimenter(s)
Diana Price
Masako Terada
Hiro Hakozaki
Microscopy product
Microscopy product ID
3687
Instrument
Biorad RTS Multiphoton
Microscopy type
multiphoton
Product type
MOSAIC
Image basename
pur2
Spatial Axis Image Size Pixel Size
X 512px 0.118 µm
Y 480px 0.118 µm
Subject
Species
mouse
Scientific name
mus musculus
Strain
C57BL/6J
Group by
NA
Treatment
none
Age
2 months
Age class
adult
Tissue section
Anatomical location
hippocampus
Microtome
Vibratome
Thickness
70 µm
Specimen description
Organ
brain
System
central nervous system
Cell type
Purkinje neuron
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
gelvatol
Lens
Nikon Plan Apo
Lens magnification
X
Numerical aperture
1.45
Specimen preparation
Protocol used
1.Sample was prepared by Diana Price from an animal perfused with 4% paraformaldehyde. Very few additional details are available regarding sample preparation.2.Masako Terada injected Lucifer Yellow into purkinje cell.3.Hiroyuki Hakozaki took image on RTS2000 with mosaic acquisition.4.Devolution has been performed by AutoDeblur version 9.3 from AutoQuant by using gold method.
Imaging product type
Type
Mosaic
Description
10% overlap between tiles
X position
4 tiles
Y position
6 tiles
Imaging product type
Type
Optical section
Cutting plane
transverse
Z resolution
0.5 um