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Reconstruction
Display image description
Purkinje neuron from mouse cerebellum injected with Lucifer Yellow and imaged using confocal microscopy. This image represents a mosaic of 4 different volumes.
Volume_dimension
2000, 2000, 80

Segmentation
Display image description
Segmentation of dendritic tree of Purkinje cell using Neurolucida

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:4*
Project: P1170
Project name
Mouse BIRN test data
Description
Neurolucida tracing of filled Purkinje neurons
Funding agency
NIH
Leader(s)
Maryann Martone
Collaborator(s)
Diana Price
Andrea Thor
Masako Terada
Hiro Hakozaki
Start date
03-01-2002
End date
unspecified
 
Experiment
Experiment ID
3
Title
Intracellular injection of Purkinje neuron
Purpose
to obtain multi resolution data for Mouse BIRN
Experimenter(s)
Andrea Thor & Diana Price
Microscopy product
Microscopy product ID
4
Instrument
Biorad Radiance 2000 Confocal
Microscopy type
confocal
Product type
Optical section series and mosaic
Image basename
e1cb4a1
Spatial Axis Image Size Pixel Size
X 1024px 0.12 µm
Y 1024px 0.12 µm
Subject
Species
mouse
Scientific name
mus musculus
Strain
C57BL/6
Treatment
none
Age
2 months
Age class
adult
Tissue section
Anatomical location
cerebellum
Microtome
vibratome
Thickness
100 µm
Specimen description
Organ
brain
System
central nervous system
Structure
dendritic tree
Map location
View location
Cell type
Purkinje neuron
Atlas coordinate
0, 0, 0,
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
gelvatol
Lens magnification
X
Numerical aperture
1.4
Imaging product type
Type
Optical section
Z resolution
0.5 um