Alternate header for print version


Reconstruction
Display image description
Single slice through a single tilt tomogram of the Node of Ranvier from mouse sciatic nerve
Full resolution image description
Tar file containing merged volume of a serial tomogram of a peripheral nerve node of Ranvier in Analyze 7.5 format
Volume_dimension
611, 611, 299
Volume scale
0.01, 0.01, 0.015

Image 2D
Display image description
Single tilt image (zero degree tilt) from the second of 3 serial single tilt series through the Node of Ranvier
Full resolution image description
tar file containing tilt images from 3 serial tilt series

Segmentation
Display image description
Manual segmentation of neuronal and glial components of the Node of Ranvier using Xvoxtrace. Objects were surfaced using Synu.
Segmentation file description
Tar file containing the surfaced objects in Synu format (*.synu) and the Viewdata file required for viewing using Synuview. The original trace file (*.trace) containing the manual contours and selected images of surface renderings are also included.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:48*
Project: P1136
Project name
Dynamics of membrane organization at the node of Ranvier
Description
Serial tomogram of a conventionally prepared peripheral nerve Node of Ranvier
Funding agency
NIH NINDS
Leader(s)
Mark Ellisman
Gina Sosinsky
Collaborator(s)
N.A
Start date
08-01-1995
End date
08-01-1995
 
Experiment
Experiment ID
28
Experiment date
12-15-1999
Title
Serial section electron tomography
Purpose
Structure and modeling
Experimenter(s)
Gina Sosinsky
Tom Deerinck
Microscopy product
Microscopy product ID
48
Instrument
JEOL 4000
Microscopy type
IVEM
Product type
single tilt
Image basename
con_node
Spatial Axis Image Size Pixel Size
X 800px 50 nm/pixel
Y 1000px 50 nm/pixel
Subject
Species
mouse
Scientific name
mus musculus
Strain
Unspecified
Age
3 days
Age class
neonate
Tissue section
Anatomical location
dorsal root
Microtome
Leica Ultracut UCT
Tissue product storage
Processed directly
Thickness
0.5 µm
Specimen description
System
peripheral nervous system
Structure
Node of Ranvier
Tissue
peripheral nerve
Cell type
Schwann cell
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
20000
Accelerating voltage
400 KeV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees