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Reconstruction
Display image description
Single slice through a double tilt tomogram of the paranodal region of the Node of Ranvier from mouse sciatic nerve
Full resolution image description
Tomogram of the paranodal region of a peripheral nerve node of Ranvier
Volume_dimension
944, 1024, 200
Volume scale
0.005, 0.005, 0.0075
Animation description
Tomographic reconstruction with annotations of the paranodal glial-axonal junction from mouse peripheral nerve, using double tilt tomography

Image 2D
Display image description
Zero tilt image from one of the double tilt series through the Node of Ranvier

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:50*
Project: P1136
Project name
Dynamics of membrane organization at the node of Ranvier
Description
Serial tomogram of a conventionally prepared peripheral nerve Node of Ranvier
Funding agency
NIH NINDS
Leader(s)
Mark Ellisman
Gina Sosinsky
Collaborator(s)
N.A
Start date
08-01-1995
End date
08-01-1995
 
Experiment
Experiment ID
30
Experiment date
10-04-2000
Title
Higher resolution tomogram of paranodal area, particularly septate junction
Purpose
Structure, localization of components
Experimenter(s)
Gina Sosinsky
Tom Deerinck
Microscopy product
Microscopy product ID
50
Instrument
JEOL 4000
Microscopy type
IVEM
Product type
double tilt
Image basename
node2X
Spatial Axis Image Size Pixel Size
X 800px 50 nm/pixel
Y 1000px 50 nm/pixel
Subject
Species
mouse
Scientific name
mus musculus
Strain
Unspecified
Age
3 days
Age class
neonate
Tissue section
Anatomical location
dorsal root
Microtome
Leica Ultracut UCT
Tissue product storage
Liquid nitrogen
Thickness
0.5 µm
Specimen description
System
peripheral nervous system
Structure
Node of Ranvier
Tissue
peripheral nerve
Cell type
Schwann cell
Imaging parameters
Type
Electron microscopy product
Magification
40000
Accelerating voltage
400 KeV
Imaging product type
Type
Double tilt
X min range
-60 degrees
X max range
60 degrees
X tilt increment
2 degrees
Y min range
-60 degrees
Y max range
60 degrees
Y tilt increment
2 degrees