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Reconstruction
Display image description
Large scale brain mosaic through level of cerebellar vermis of a wild type mouse labeled for alpha synuclein (green) and a nuclear stain (blue). Final montage is a projection through 5 z planes.
Full resolution image description
Large scale brain mosaic through level of cerebellar vermis of a wild type mouse labeled for alpha
Volume_dimension
16445, 14780, 1
Volume scale
0.24, 0.24, 3

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:53*  Cite 
Project: P1187
Project name
Correlated Imaging Approaches and Multiscale Databases for Research in Parkinson's Disease
Description
characterization of a mouse model of human alpha synuclein overexpressor
Funding agency
The Branfman Family Foundation
Leader(s)
Diana Price
Collaborator(s)
M.H. Ellisman; M. Martone; G.A. Johnson; E. Masliah
Start date
09-01-2002
End date
09-01-2002
 
Experiment
Experiment ID
18
Experiment date
03-07-2003
Title
Alpha-synuclein immunolabeling for large-scale mapping study
Purpose
to determine the distribution of alpha-synuclein immunolabeling in wildtype tissue
Experimenter(s)
Diana Price
Microscopy product
Microscopy product ID
53
Instrument
Biorad RTS Multiphoton
Microscopy type
multiphoton
Product type
optical section series/mosaic
Image basename
102003b
Spatial Axis Image Size Pixel Size
X 512px 0.27 µm
Y 480px 0.27 µm
Subject
Species
mouse
Scientific name
mus musculus
Strain
C57BL/6 * DBA/2
Group by
genetic manipulation
Treatment
NA
Age
291 days
Age class
adult
Tissue section
Anatomical location
cerebellum
Microtome
vibratome
Thickness
80 µm
Specimen description
Organ
brain
System
central nervous system
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
gelvatol
Lens
Nikon Plan Fluor
Lens magnification
X
Numerical aperture
1.4
Imaging product type
Type
Optical section
Z resolution
3 um