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Reconstruction
Display image description
Large scale mosaic of mouse cerebellum reconstructed through automated acquisition and stitching of over 1700 individual tiles, triple labeled for GFAP (Red), Connexin 43 (Green) and DAPI (Blue), and imaged using multiphoton microscopy. The contrast and brightness was adjusted on the thumbnail image for display purposes and is different on the downloadable and Zoomify data.
Full resolution image description
1,776 tiled images were stitched together to reconstruct this single, high-resolution mosaic image of the cerebellum within a sagittal section of the left hemisphere of the mouse brain. Red is Rhodamine RedX labeling of Chicken anti-GFAP. Green is Alexafluor488 labeling of Rabbit anti-Connexin43. Blue is DAPI staining of all nuclei.
File format
TIFF
Volume_dimension
17440, 21160, 3
Volume scale
0.24, 0.24, 3

Image 2D
Display image description
A representative tile from a large mosaic: this thumbnail image is a projection of 4 Z-slices from 3 fluorescent channels acquired from brain tissue using a 2-photon confocal microscope. Red is Rhodamine RedX labeling of Chicken anti-GFAP. Green is Alexafluor488 labeling of Connexin43. Blue is DAPI staining of all nuclei.
Full resolution image description
This raw data file contains 7,104 3-color images (37x48x4) that make up the 1,776 tiles of this brain montage. A 50 micron thick sagittal section of the left hemisphere of the mouse brain was labeled with an antibody against Connexin43 protein and an antibody against GFAP protein. The nuclei of the brain tissue were labelled with DAPI staining. The area of the entire cerebellum was imaged using a 2-photon confocal microscope.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:6624*  Cite 
Project: P2029
Project name
Pannexin protein expression in mouse brain tissue
Description
A neuroinformatics approach to pannexin expression, localization, and interaction with other brain proteins. This research aims to examine brain tissue at high resolution and, in combination with developing neuronformatics tools, to probe for binding partners and signalling partners to investigate the pannexin "interactome". Data submissions for this project will consist of wide-scale high resolution confocal mosaic images of triple-labeled whole mouse brain sections. Pannexin 1 expression will be compared to that of pannexin 2 as well as the connexins. Other putative interaction partners of interest include the P2 purinergic receptors and components of the cytoskeleton.
Leader(s)
Dr. Angela Cone
Collaborator(s)
Dr. Gina Sosinsky
Dr. Maryann Martone
Start date
11-06-2008
End date
unspecified
 
Experiment
Experiment ID
6616
Experiment date
12-22-2008
Title
Panx1 compared to Cx43 and astrocyte labelling
Purpose
Panx1 compared to Cx43 and astrocyte labelling
Experimenter(s)
Dr. Angela Cone
Microscopy product
Microscopy product ID
6624
Instrument
RTS2000
Microscopy type
multiphoton
Product type
MOSAIC
Image basename
12222008Slide3
Spatial Axis Image Size Pixel Size
X 512px 0.24 um/pixels
Y 480px 0.24 um/pixels
Y 4px
Subject
Species
mouse
Scientific name
Mus musculus
Strain
unknown
Age class
unknown
Tissue section
Anatomical location
b
Microtome
vibratome sectioned
Tissue product storage
cryoprotectant
Thickness
50 µm
Specimen description
Organ
brain
System
central nervous system
Map location
View location
Atlas coordinate
0, 0, 0,
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
gelvatol
Lens
Nikon
Lens magnification
X
Numerical aperture
1.45
Specimen preparation
Protocol used
Fixation: 4% paraformaldehydeRabbit Cx43 antibody (Sigma) 1/600Chicken GFAP antibody (Neuromics) 1/50072hour incubation at 4CSecondaries:Donkey anti rabbit-Al488Donky anti-chicken-RRX2.5hour incubation at room tempDAPI
Imaging product type
Type
Mosaic
Description
Cerebellum labeled for Connexin43 and GFAP
X position
37 tiles
Y position
48 tiles