Alternate header for print version


Reconstruction
Display image description
EM analysis using SBEM of presynaptic nerve terminals found in the hippocampus CA1 (stratum radiatum) in non-Tg mice.
Full resolution image description
EM analysis using SBEM of presynaptic nerve terminals found in the hippocampus CA1 (stratum radiatum) in non-Tg mice.
File format
MRC

Segmentation
Segmentation file description
Three-dimensional volume segmentation of same SBEM dataset

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:67820*
Project: P20094
Project name
Molecular and cellular investigations of alpha-synuclein in Parkinson pathogenesis
Description
The goal of this project is to better understand the role of alpha-synuclein in the progression of PD by applying labeling systems developed at NCMIR to track, at multiple resolution scales, the distribution and trafficking of these proteins associated with Parkinson's disease.
Leader(s)
Daniela Boassa
Collaborator(s)
Monica L. Berlanga
Mary Ann Yang
Masako Terada
Junru Hu
Eric A. Bushong
Minju Hwang
Eliezer Masliah
Julia M. George
and Mark H. Ellisman
Start date
unspecified
End date
unspecified
 
Experiment
Experiment ID
9643
Title
Expression of human-alpha-synuclein in transgenic mice
Purpose
Investigate distribution of over-expressed alpha-synuclein in transgenice mice
Experimenter(s)
Daniela Boassa
Microscopy product
Microscopy product ID
67820
Instrument
3View
Microscopy type
Serial Block Face SEM
Product type
SERIAL SECTION
Image basename
M82_control_Vol1
Subject
Species
Mouse
Scientific name
Mus
Strain
NA
Age
17 months
Age class
Adult
Tissue section
Anatomical location
Brain
Specimen description
Organ
Brain
System
central nervous system
Imaging parameters
Type
Electron microscopy product
Magification
2000
Accelerating voltage
2.5 KV
Specimen preparation
Protocol used
Transgenic (Tg) mice (n = 3) overexpressing human wild-type -synuclein (PDGFhuman -synuclein Tg mice, line D, from Masliah et al., 2000) and their littermate wild-type controls (n = 3), 10- to 17- month-old males, were used in this study.
Imaging product type
Type
Serial section
Description
Serial block face scanning EM
Z resolution
70 nm