Specimen Preparation Information
Microscopy product ID: 1023
Image basename: 3wk-ly2
|
Specimen Preparation
|
|
Tissue group type
|
NA
|
|
Ext file name
|
NA
|
| Protocol used |
|
|
Microscopy Product Information
Microscopy product ID: 1023
Image basename: 3wk-ly2
Microscopy product
|
| Microscopy product id |
1023 |
| Image basename |
3wk-ly2 |
| Instrument |
Biorad Radiance2000
|
| X Size |
1024
|
| Y Size |
1024
|
| Z Size |
|
| X Resolution |
0.051389 um/pixel
|
| Y Resolution |
0.051389 um/pixel
|
| Z Resolution |
|
| Create date |
|
| PURL |
NA
|
| Product type |
optical section series
|
| Microscope type |
single photon confocal
|
| Session name |
|
| Grid box count |
|
| Grid info. |
N/A
|
| Slide info. |
|
| Plane count |
0
|
| SRB ID |
0
|
|
Back to top
|
Imaging Product Types
Microscopy product ID: 1023
Image basename: 3wk-ly2
|
Optical section series
|
| Optical PSF file |
|
| Descriptions |
|
| Optical Z resolution |
0.2 um |
| Cutting plane |
|
|
Back to top
|
|
Through focus series
|
| Descriptions |
|
| Through PSF file |
|
| Z step |
|
|
Back to top
|
Specimen Description
Microscopy product ID: 1023
Image basename: 3wk-ly2
|
Specimen description (1023, 3wk-ly2)
|
| System |
central nervous system
|
| Tissue |
|
| Organ |
brain |
| Region |
hippocampus |
| Subregions |
CA1, stratum radiatum
|
| Cell type |
protoplasmic astrocyte |
| Cell id |
|
| Structure |
|
| Atlas coordinates |
|
| Atlas |
|
| Map location |
|
| Notes |
|
Imaging Parameters
Microscopy product ID: 1023
Image basename: 3wk-ly2
|
Light microscopy product (1023, 3wk-ly2)
|
| Coverslip thickness |
1.0 um
|
| Lens |
Nikon |
| Lens magnification |
60.0 X |
| Numerical aperture |
1.4 |
| Immersion medium |
oil |
| Mounting medium |
gelvatol |
| Refractive index |
1.5 |
|
Back to top
|
LM channels
| Confocal channel (10124) |
| Filter used |
|
| Fluorophor |
Lucifer Yellow |
| Color |
Green
|
| Excitation wave length |
428.0 nm |
| Emission wave length |
536.0 nm |
| Laser type |
Krypton-Argon |
| Notes |
|
Specimen preparations
View complete protocol
|
|
Back to top
2D Image Information
Microscopy product ID: 1023
Image basename: 3wk-ly2
Reconstruction Information
Microscopy product ID: 1023
Image basename: 3wk-ly2
|
Reconstruction description
|
|
Reconstruction description
|
|
|
Full-resolution reconstruction file
|
|
|
Down-sampled version of the reconstruction file
|
|
| Image map file |
|
|
Reconstruction file format
|
|
| Volume dimension |
|
| Voxel scale |
|
|
|
|
| Animation
|
|
No animation available.
|
|
|
 |
| Data files |
| Action |
File type |
File size |
Description |
|
Download
|
512x512 image
|
Unknown
|
Optical section series through a protoplasmic astrocyte in rat hippocampal area CA1 intracellulaly injected Lucifer Yellow and imaged with confocal microscopy
|
|
Download
|
Reconstruction data file
|
85.57MB
|
Not available
|
|
Description: Optical section series through a protoplasmic astrocyte in rat hippocampal area CA1 intracellulaly injected Lucifer Yellow and imaged with confocal microscopy
Description: Optical section series through a protoplasmic astrocyte in rat hippocampal area CA1 intracellulaly injected Lucifer Yellow and imaged with confocal microscopy
|
Segmentation Information
Microscopy product ID: 1023
Image basename: 3wk-ly2
|
|
| Message |
|
No segmentation data is available for this data set yet.
|