Alternate header for print version


Image 2D
Display image description
SIngle slice from a through focus series through a medium spiny neuron from the dorsal neostriatum of a wild type mouse. Neuron was stained by intracellular injection of Lucifer Yellow, followed by photooxidation. The flocculent material surrounding the neuron likely represents photoconverted mitochondria or else debris generated by the photoconversion process.
Full resolution image description
Zip file containing through focus series in original BioRad PIC format and also converted to multiimage tiff format
Animation description
Animation stepping through a through-focus series of a medium spiny neuron from the dorsal neostriatum of a wild type mouse. Neuron was stained by intracellular injection of Lucifer Yellow, followed by photooxidation. The flocculent material surrounding the neuron likely represents photoconverted mitochondria or else debris generated by the photoconversion process.

Segmentation
Display image description
Manual tracing of dendrites using Neurolucida. Because of the low magnification and photoconversion debris, the processes were sometimes difficult to follow.
Segmentation file description
Zip file containing Neurolucida trace file in ascii format (050803b_finaltrace.ASC), along with the output in VRML format. Summary files of measurements generated by Neuroexplorer for each of the parts traced are also included.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3382*  Cite 
Project: P1207
Project name
Correlative microscopic characterization of dendritic spines in a transgenic mouse model of hyperdopaminergia: The dopamine transporter knockout mouse
Description
Multiscale characterization of DAT KO transgenic mouse
Funding agency
NIH
Leader(s)
Diana Price
Collaborator(s)
Aki Laakso
Michele Cyr
Maryann Martone
Naoko Yamada
Andrea Thor
Monica Berlanga
Start date
01-01-2003
End date
unspecified
 
Experiment
Experiment ID
19
Experiment date
04-22-2003
Title
P1207 Experiment 5
Purpose
EMT reconstructions of medium spiny neuron dendrites
Experimenter(s)
Diana Price
Masako Terada
Andrea Thor
Microscopy product
Microscopy product ID
3382
Instrument
Bio-Rad Radiance 2000
Microscopy type
confocal
Product type
THROUGH FOCUS SERIES
Image basename
050803C
Spatial Axis Image Size Pixel Size
X 1024px 0.19 um/pixels
Y 1024px 0.19 um/pixels
Subject
Species
mouse
Scientific name
Mus Musculus
Strain
C57BL/129SvJ
Group by
genetic manipulation
Treatment
NA
Age
7 months
Age class
adult
Tissue section
Anatomical location
neostriatum 050803C
Microtome
Vibratome
Tissue product storage
P1207Slidebox1
Thickness
100 µm
Specimen description
Organ
brain
System
central nervous
Tissue
striatum
Map location
View location
Cell type
medium spiny neuron
Atlas coordinate
0, 0, 0,
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
resin
Lens
Nikon Plan Apo
Lens magnification
X
Numerical aperture
1.4
Imaging product type
Type
Through focus
Description
transmitted light z series through photoconverted medium spiny dendride
Z step
.5 microns
Psf file
050803C