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Reconstruction
Display image description
A single slice taken from the recontructed slice-by-slice volume of a Caulobacter Crescentus specimen. This image is taken from Z slice #271.
Full resolution image description
The final reconstructed slice-by-slice volume of a caulobacter crescentus specimen. The tar file contains a single .mrc file stack with dimensions 1803, 2889, 271 in the xyz in a floating point (mode 2) file format. The reconstructed volume has been trimmed and has had a median filter via IMOD applied using default settings.
Volume_dimension
1803, 2889, 271
Animation description
A .mpg file showing the slice by slice reconstruction of a Caulobacter Crescentus specimen.

Image 2D
Display image description
The zero degree tilt image of a Caulobacter Crescentus specimen.
Full resolution image description
.tar file containing all files necessary to make a reconstructed volume using the quadratic reconstruction algorithm usin TxBR. In addition, a caulo3_info.txt ascii file is included outlining the details of this dataset.
Animation description
a .mpg file of an aligned tilt series. Movie starts at the 0 degree image, move to each extreme angle and then back to 0 degrees.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3646*  Cite 
Project: P1220
Project name
Caulobacter
Description
Tomography of Caulobacter crescentus
Funding agency
Department of Energy
Leader(s)
Lucy Shapiro
Harley McAdams
Collaborator(s)
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Start date
07-01-2003
End date
unspecified
 
Experiment
Experiment ID
3374
Experiment date
05-12-2004
Title
Tomographic reconstruction of caulobacter
Purpose
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3646
Instrument
JEOL 4000 #1
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
caulo3
Spatial Axis Image Size Pixel Size
X 2242px 1 nm/pixels
Y 3340px 1 nm/pixels
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus (NA1000)
Strain
NA1000
Treatment
None
Age class
N/A
Tissue section
Thickness
0.5 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
30000
Accelerating voltage
400 kV
Notes
Caulobacter Crescentus tilt series. Gold fiducial markers are 10 and 20 nm in diameter.
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees
Notes
Caulobacter Crescentus Tomogram imaged on a 400kV JEOL 4000. Images shot by Ying Jones