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Reconstruction
Display image description
A 512 by 512 image from the slice by slice reconstruction of a caulobacter crescentus specimen.
Full resolution image description
a .tar file containing the final reconstructed volume of a caulobacter crescentus specimen. Reconstructed volume is an IMOD .mrc file.
Volume_dimension
2242, 1734, 350
Animation description
a .mpg movie of the maximum intensity projection derived from a caulobacter crescentus specimen.

Image 2D
Display image description
Caulobacter Crescentus specimen from the tilt series shot on a 400kV microscope. This image was taken from the "a" stack at the 0 degree tilt.
Full resolution image description
a .tar file containing all files needed for reconstruction using the TxBR reconstruction package. Both the a and b stacks, rawtlt file, and TxBR files are included in the .tar file.
Animation description
a .mpg file containing the tilt series. The series shown here is only aligned using cross correlation algorithms.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3660*  Cite 
Project: P1220
Project name
Caulobacter
Description
Tomography of Caulobacter crescentus
Funding agency
Department of Energy
Leader(s)
Lucy Shapiro
Harley McAdams
Collaborator(s)
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Start date
07-01-2003
End date
unspecified
 
Experiment
Experiment ID
3374
Experiment date
05-12-2004
Title
Tomographic reconstruction of caulobacter
Purpose
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3660
Instrument
JEOL 4000 #1
Microscopy type
IVEM
Product type
DOUBLE TILT
Image basename
caulodouble2
Spatial Axis Image Size Pixel Size
X 2242px
Y 3340px
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus (NA1000)
Strain
NA1000
Treatment
None
Age class
N/A
Tissue section
Thickness
0.5 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
30000
Accelerating voltage
400 kV
Specimen preparation
Protocol used
1) Prefix with 1% Acreolin and 2.5% Glutaradehyde2) High Pressure Freezing 3) Free Substitution with 2% Osmium and .1% UA4) Durcupan embedded
Imaging product type
Type
Double tilt
X min range
-60 degrees
X max range
60 degrees
X tilt increment
2 degrees
Y min range
-58 degrees
Y max range
60 degrees
Y tilt increment
2 degrees
Description
Caulobacter Crescentus, 0.5 um thick, 400kv