Alternate header for print version


Image 2D
Display image description
Z projection through a through-focus series of a medium spiny neuron from a dopamine transporter knock out mouse, iinjected with Lucifer Yellow and then photoconverted.
Full resolution image description
Zip archive containing original through focus series in BioRadPIC format (DATKOK.PIC) and in multiimage tiff format (DATKOKTLseries.tif).
Animation description
Animation through the through focus series of a medium spiny neuron from a dopamine transporter knock out mouse, iinjected with Lucifer Yellow and then photoconverted. Contrast has been adjusted compared to the original data and the size adjusted for display purposes. The original data is available for download.

Segmentation
Display image description
Manual tracing of dendrites using Neurolucida. Spines were traced but these were difficult to see, so the number may not be accurate.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3693*  Cite 
Project: P1207
Project name
Correlative microscopic characterization of dendritic spines in a transgenic mouse model of hyperdopaminergia: The dopamine transporter knockout mouse
Description
Multiscale characterization of DAT KO transgenic mouse
Funding agency
NIH
Leader(s)
Diana Price
Collaborator(s)
Aki Laakso
Michele Cyr
Maryann Martone
Naoko Yamada
Andrea Thor
Monica Berlanga
Start date
01-01-2003
End date
unspecified
 
Experiment
Experiment ID
20
Experiment date
01-09-2003
Title
P1207 Experiment 1
Purpose
EMT reconstructions of medium spiny neuron dendrites
Experimenter(s)
Diana Price
Masako Terada
Andrea Thor
Microscopy product
Microscopy product ID
3693
Instrument
Biorad 1024 MRC confocal
Microscopy type
transmitted light
Product type
THROUGH FOCUS SERIES
Image basename
DATKOK
Spatial Axis Image Size Pixel Size
X 1024px 0.248 um/pixels
Y 1024px 0.248 um/pixels
Subject
Species
mouse
Scientific name
mus musculus
Strain
C57BL/129SvJ
Group by
genetic manipulation
Treatment
Knockout of dopamine transporter
Age
6 months
Age class
adult
Tissue section
Anatomical location
neostriatum DATKOK/Q
Microtome
vibratome
Tissue product storage
Slide Box #1
Thickness
100 µm
Specimen description
Organ
brain
System
central nervous system
Map location
View location
Cell type
medium spiny neuron
Atlas coordinate
0, 0, 0,
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
epoxy
Lens magnification
X
Imaging product type
Type
Through focus
Z step
.54 um
Notes
Zoom = 1; Kalman = 4