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Reconstruction
Display image description
A maximum intensity projection of a Caulobacter crescentus specimen at 0 degree tilt.
Full resolution image description
This .tar file contains two .mrc reconstructed volumes: cb1_full.rec and cb1.rec. cb1_full.rec is the full, untrimmed (and unflipped) volume. cb1.rec is the trimmed slice by slice volume used for subsequent analysis.
Volume_dimension
993, 1582, 101
Volume scale
0.001, 0.001, 0.001
Animation description
a .mpg animation movie of a maximum intensity projection of Caulbacter crescentus, rotating about the y-axis.

Image 2D
Display image description
A Caulobacter crescentus specimen from the aligned tilt series at 0 degree tilt.
Full resolution image description
A .tar file containing all files necessary for a slice by slice volume reconstruction using IMOD. Some files included are the .st, .preali, .ali, .fid, .rawtlt and a notes.txt file listing some procedures used during the reconstruction process.
Animation description
a .mpg file format movie of a Caulobacter crescentus. Movie involves the aligned tilt series.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3700*  Cite 
Project: P1220
Project name
Caulobacter
Description
Tomography of Caulobacter crescentus
Funding agency
Department of Energy
Leader(s)
Lucy Shapiro
Harley McAdams
Collaborator(s)
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Start date
07-01-2003
End date
unspecified
 
Experiment
Experiment ID
3439
Experiment date
04-01-2007
Title
Tomographic reconstruction of caulobacter
Purpose
To produce a series of tomographic reconstructions of the normal morphology of caulobacter using traditional fixing methods.
Experimenter(s)
Tom Deerinck
Guido Gaietta
Microscopy product
Microscopy product ID
3700
Instrument
JEOL 4000 #1
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
cb1
Spatial Axis Image Size Pixel Size
X 4020px 0.556 nm/pixels
Y 4020px 0.556 nm/pixels
Subject
Species
Caulobacter crescentus
Scientific name
Caulobacter crescentus (Snc04)
Strain
Snc04
Age class
N/A
Tissue section
Anatomical location
N/A
Tissue product storage
N/A
Thickness
0.4 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
CCD
Magification
40000
Specimen preparation
Protocol used
Induced for 1 hour at 30 degrees CLabeled in M2G medium for 2 hours at 30 degrees C (in the presence of 1.8 micromolar ReAsH-EDT2) and photoconvertedSamples were fixed with 2.5% glutaraldehyde with 1% acrolein in 0.05M sodium cacodylate pH 7.4 with 3 mM CaCl2 and 1 % sucrose followed by conventional osmium tetroxide/potassium ferrocyanide post-fixation, dehydration and embedding.
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees