Alternate header for print version


Reconstruction
Display image description
Wide field mosaic of mouse hippocampus immunolabeled for pannexin 1 (green), GFAP (blue) and connexin 43 (red). The section was also counterstained with DAPI (not shown).
Full resolution image description
176 tiles (16x11) were digitally stitched together to generate a 3D mosaic montage of the mouse hippocampus labeled with three different antibodies (Green = Pannexin1 protein, Red = Connexin43 protein, Blue = GFAP protein within astrocytes). This zipped tar file contains 24 reconstructed mosaic images (file names labeled 00-23), one for each of the 24 optical Z-sections that were acquired with a step distance of 0.5 microns. Additionally, the zip file contains a mosaic 2D maximum intensity projection of all 24 Z-sections (file name containing "proj").
Downsampled image description
This image is a mosaic 2D maximum intensity projection of all 24 Z-sections. Voxel scale for this flattened projection of the mosaic hippocampus is 0.41 x 0.41 x 11.5 microns/pixel. The tiles in this image have been normalized, but the colors in the resulting image have not been adjusted.
File format
TIFF
Volume_dimension
14914, 10341, 24
Volume scale
0.41, 0.41, 11.5

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:6674*  Cite 
Project: P2029
Project name
Pannexin protein expression in mouse brain tissue
Description
A neuroinformatics approach to pannexin expression, localization, and interaction with other brain proteins. This research aims to examine brain tissue at high resolution and, in combination with developing neuronformatics tools, to probe for binding partners and signalling partners to investigate the pannexin "interactome". Data submissions for this project will consist of wide-scale high resolution confocal mosaic images of triple-labeled whole mouse brain sections. Pannexin 1 expression will be compared to that of pannexin 2 as well as the connexins. Other putative interaction partners of interest include the P2 purinergic receptors and components of the cytoskeleton.
Leader(s)
Dr. Angela Cone
Collaborator(s)
Dr. Gina Sosinsky
Dr. Maryann Martone
Start date
11-06-2008
End date
unspecified
 
Experiment
Experiment ID
6616
Experiment date
12-22-2008
Title
Panx1 compared to Cx43 and astrocyte labelling
Purpose
Panx1 compared to Cx43 and astrocyte labelling
Experimenter(s)
Dr. Angela Cone
Microscopy product
Microscopy product ID
6674
Instrument
Olympus Fluoview1000
Microscopy type
LASER SCANNING CONFOCAL
Product type
MOSAIC
Image basename
12222008Slide1Hip
Spatial Axis Image Size Pixel Size
X 1024px 0.41 um/pixels
Y 1024px 0.41 um/pixels
Y 24px
Subject
Species
mouse
Scientific name
Mus musculus
Strain
unknown
Age class
unknown
Tissue section
Anatomical location
b
Microtome
vibratome sectioned
Tissue product storage
cryoprotectant
Thickness
50 µm
Specimen description
Organ
Brain
System
central nervous system
Map location
View location
Atlas coordinate
0, 0, 0,
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
gelvatol
Lens
Olympus PlanApo 60X oil
Lens magnification
X
Numerical aperture
1.42
Notes
acone
Specimen preparation
Protocol used
Rabbit Cx43 antibody (Sigma) 1/600Guinea pig GFAP antibody (discontinued) 1/600Chicken Panx1 antibody (4515) 1/25072hour incubation at 4C.Secondaries:DAR-Al647DAGP-RRXDAC-FITC2.5hours incubation at room temperature.
Imaging product type
Type
Mosaic
Description
Hippocampus region of 50 micron sagittal section of mouse brain
X position
16 tiles
Y position
11 tiles