Alternate header for print version


Reconstruction
Display image description
TIFF thumbnail taken from the middle slice of a mouse retina imaged with the 3view.
Full resolution image description
Tar file containing 3view reconstruction of mouse retina images. MRC file is 8bit and has been aligned with some images deleted. Also contains a folder with all original alignment files.
Downsampled image description
Downsampled MRC of 3view reconstruction of mouse retina, bin 8. Dataset binned using 2D downsampling instead of 3D downsampling due to the nature of the 3view data.
File format
MRC
Volume_dimension
14688, 8000, 206
Volume scale
0.013, 0.013, 0.07
Animation description
MP4 animation of computated slices through a mouse retina using the 3view.

Image 2D
Display image description
Single slice from a serial section series through the mouse retina taken with serial block face SEM.
Full resolution image description
Tar file containing all raw DM3 files from the 3view. We recommend downloading the reconstruction file rather than the raw data, as it has been cleaned up considerably.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:7742*  Cite 
Project: P2044
Project name
Study of the fine structure of the visual system
Leader(s)
Keun-Young Kim
Collaborator(s)
Mark Ellisman
Start date
06-22-2009
End date
unspecified
 
Experiment
Experiment ID
7734
Experiment date
02-08-2010
Title
Ultrastructural Characterization of the Mouse Optic nerve Head and Retina
Purpose
The purpose of this study was to investigate the ultrastructure of the mouse optic nerve head and retina.
Experimenter(s)
Keun-Young Kim
Eric Bushong
Microscopy product
Microscopy product ID
7742
Instrument
Gatan 3View system on FEI Quanta microscope
Microscopy type
Serial Block Face SEM
Product type
SERIAL SECTION
Image basename
mouse_retina_v2
Spatial Axis Image Size Pixel Size
X 4096px 17 nm/pixels
Y 4096px 17 nm/pixels
Subject
Species
mouse
Scientific name
mus musculus
Strain
C57BL/6
Age
9 months
Age class
Adult
Tissue section
Anatomical location
Retina
Microtome
Leica ultramicrotome
Tissue product storage
Durcupan
Thickness
30 µm
Specimen description
Organ
eye
System
central nervous system
Imaging parameters
Type
Electron microscopy product
Magification
0
Imaging product type
Type
Serial section
Description
Gatan 3-view sectioning