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Reconstruction
Display image description
Conputed slice through a tomographic reconstruction of the mitochondrial adherens complex in the Calyx of Held of an adult cat. The specific mitochondrion is shown was segmented and can be found in the segmentation section of this record.
Full resolution image description
Zip file containing the reconstruction file (king_filt.img/hdr) in Analyze 7.5 format (king_vol.zip) that was used to produce the segmentation for this reconstruction.
File format
Analyze 7.5
Volume_dimension
375, 600, 250
Animation description
Slices through the reconstructed volume

Image 2D
Display image description
Zero degree tilt image of a 0.5 micron section of a mitochondrion and a mitochondrion-associated adherens complex in the Calyx of Held of the adult cat
Full resolution image description
Tar.gz file (tilt_images.tar.gz) of the aligned tilt images of the the Mitochondrion-associated Adherens Complex (MAC) at the calyx of Held nerve terminal showing the location of mitochondria relative to the active sites and adherens junctions.
Animation description
Animation of aligned tilt images of the Mitochondrion-associated Adherens Complex (MAC) at the calyx of Held nerve terminal showing the location of mitochondria relative to the active sites and adherens junctions.

Segmentation
Display image description
Segmentation of mitochondrion outer and inner membranes, cristae
Segmentation file description
Tar.gz file (segment_data.tar.gz) of the segmentation of a mitochondrion from the Mitochondrion-associated Adherens Complex (MAC) at the calyx of Held nerve terminal showing the location of mitochondria relative to the active sites and adherens junctions. Shows outer membrane, inner membrane and cristae.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3487*  Cite 
Project: P1223
Project name
Electron tomography of mitochondrial adherens complex in the Calyx of Held
Description
Reconstruction of mitochondrial associated adherens complex in the Calyx of Held synapse using electron tomography. The adherens complex is an elaborate cytoskeletal superstructure connecting a subset of mitochondria to the presynaptic membrane near active zones. Note: several additional reconstructions from this project are available from Dr. George Spirou upon request.
Funding agency
National Institutes of Health
Leader(s)
Guy Perkins
Collaborator(s)
George Spirou
Start date
06-01-2003
End date
06-01-2003
 
Experiment
Experiment ID
3384
Title
MAC
Purpose
purpose
Experimenter(s)
Guy Perkins
Microscopy product
Microscopy product ID
3487
Instrument
JEOL4000EX
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
king
Subject
Species
cat
Scientific name
felis catus
Age class
Adult
Tissue section
Anatomical location
Medial Nucleus of the Trapezoidal Body
Thickness
0.5 µm
Specimen description
Organ
brain
System
central nervous system
Structure
mitochondrion
Imaging parameters
Type
Electron microscopy product
Recording medium
Tietz 4 K CCD
Magification
20000
Accelerating voltage
400 keV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees