Alternate header for print version


Reconstruction
Display image description
Computed slices through a tomographic reconstruction of mitochondria in a retina rod cell of an adult mouse.
Full resolution image description
Zip file containing the tomographic volume in Analyze 7.5 format (gap18_sub.hdr/img)
File format
Analyze 7.5
Volume_dimension
350, 600, 130
Animation description
Animation through the computed slices of a tomographic reconstruction of mitochondria in a retina rod cell of an adult mouse. Mitochondrial membranes and crista were hand segmented and surface rendered.

Image 2D
Display image description
Single image from a tilt series taken of mitochondria in a retinal rod cell from an adult rat. The entire tilt series is available for download. Note that contrast is reversed so that electron dense structures appear bright.
Full resolution image description
Unaligned tilt series in IMOD format (gap18.st)

Segmentation
Display image description
Manual segmentation of mitochondrial membranes and crista using Xvoxtrace 2.13
Segmentation file description
File generated by Xvoxtrace containing the manual contours. The volume file used for tracing is available under the reconstruction section.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:8747*  Cite 
Project: P1209
Project name
Cone and Rod mitochondria
Description
In situ structures of mitochondria in rods and cones
Leader(s)
Don Fox
University of Houston
Collaborator(s)
Guy Perkins
Start date
09-01-2001
End date
09-01-2001
 
Experiment
Experiment ID
33
Experiment date
07-01-2001
Title
Cone and rod mitochondria: electron tomography
Purpose
electron tomography of cone mitochondria
Experimenter(s)
Guy Perkins
Microscopy product
Microscopy product ID
8747
Instrument
JEOL4000EX
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
gap18
Subject
Species
mouse
Scientific name
mus musculus
Strain
C57BL/6
Treatment
none
Age class
adult
Tissue section
Anatomical location
retina
Specimen description
Organ
eye
System
central nervous system
Structure
mitochondrion
Cell type
retina rod cell
Imaging parameters
Type
Electron microscopy product
Magification
0
Accelerating voltage
400 KeV
Specimen preparation
Protocol used
These specimens were prepared similarly to those described in the Perkins et al. 2003 study (see project info), although the reconstruction did not appear in this paper.