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Reconstruction
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Maximum intensity projection of an optical section series of a protoplasmic astrocyte injected with Lucifer Yellow in rat hippocampal area CA1

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Project: P1230
Project name
Astrocyte Development
Description
Postnatal development of protoplasmic astrocytes
Funding agency
NIH
Leader(s)
Eric Bushong
Collaborator(s)
Maryann Martone
Mark Ellisman
Start date
02-01-2002
End date
unspecified
 
Experiment
Experiment ID
8
Experiment date
02-28-2002
Title
Intracellular injection of an astrocyte
Purpose
To study the development of astrocytes in situ
Experimenter(s)
Eric Bushong
Microscopy product
Microscopy product ID
11
Instrument
Biorad Radiance 2000 Confocal
Microscopy type
confocal
Product type
optical section series
Image basename
3wk-ly27
Spatial Axis Image Size Pixel Size
X 1024px 0.05873 µm
Y 1024px 0.05873 µm
Subject
Species
rat
Scientific name
rattus norvegicus
Strain
Sprague Dawley
Group by
age
Treatment
3 week old
Age
3 weeks
Age class
juvenile
Tissue section
Anatomical location
hippocampus
Microtome
vibratome
Thickness
100 µm
Specimen description
Organ
brain
System
central nervous system
Cell type
protoplasmic astrocyte
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
gelvatol
Lens magnification
X
Numerical aperture
1.4
Imaging product type
Type
Optical section
Z resolution
0.25 um