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Specific labeling of Panx2-4Cys and electron tomography highlight Panx2 distribution in the membrane of intracellular vesicles. MDCK cells stably expressing Panx2-4Cys were stained with ReAsH-EDT2, fixed and imaged with a confocal microscope before photooxidation. MDCK cells stably expressing Panx2-4Cys were stained with ReAsH-EDT2, fixed and imaged with a confocal microscope before photooxidation.
Full resolution image description
Specific labeling of Panx2-4Cys and electron tomography highlight Panx2 distribution in the membrane of intracellular vesicles. MDCK cells stably expressing Panx2-4Cys were stained with ReAsH-EDT2, fixed and imaged with a confocal microscope before photooxidation. MDCK cells stably expressing Panx2-4Cys were stained with ReAsH-EDT2, fixed and imaged with a confocal microscope before photooxidation.
File format
MRC
Volume_dimension
4933, 4933, 611
Image 2D
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Single tilt image (93.3 degree tilt) from the second of 3 serial single tilt series
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tar file containing tilt images from 3 serial tilt series
Segmentation
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Vesicle containing Panx2 proteins
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Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:48720*  Cite 
Project: P2010
Project name
Pannexin Distribution in the Adult Mouse Brain
Description
Investigation of pannexins expression and distribution in the adult mouse brain.
Leader(s)
Daniela Boassa
Start date
unspecified
End date
unspecified
 
Experiment
Experiment ID
9770
Title
High resolution imaging of Panx2 protein
Purpose
To image at high resolution by tomography the pannexin2 channels.
Experimenter(s)
Daniela Boassa
Microscopy product
Microscopy product ID
48720
Instrument
JEOL 4000#1
Microscopy type
IVEM
Product type
MULTIPLE TILT
Image basename
pan_30k
Spatial Axis Image Size Pixel Size
X 4933px
Y 4933px
Y 611px
Subject
Species
Madin-Darby Canine Kidney Epithelial Cells
Scientific name
Canis
Strain
NA
Group by
Cell type
Treatment
Stable cell line expressing Panx2-4C. Labeled with ReAsH then processed for photooxidation and EM.
Age class
NA
Specimen description
Organ
brain
System
central nervous system
Structure
Vesicle
Cell type
MDCK cell
Imaging parameters
Type
Electron microscopy product
Recording medium
4K CCD
Magification
30000
Accelerating voltage
400 kV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees