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Reconstruction
Display image description
Single computed slice through a tomographic reconstruction of a synaptic contact in hippocampal area CA1 neuropil, from tissue that was prepared by a chemical fixation followed by high pressure freezing.
Full resolution image description
Tar file containing TxBR (projective) combined tomographic volume of high pressure frozen hippocampus tissue, showing a synaptic contact.
Volume_dimension
2242, 3340, 350
Volume scale
0.0008, 0.0008, 0.0008
Animation description
Animation of a tomographic reconstruction of a synaptic contact in hippocampal area CA 1 neuropil, from tissue that was prepared by a chemical fixation followed by high pressure freezing.

Image 2D
Display image description
Zero degree tilt electron micrograph from a tilt series of a synaptic contact in hippocampal area CA1 neuropil imaged using intermediate voltage electron microscopy, from tissue that was prepared by a chemical fixation followed by high pressure freezing.
Full resolution image description
Tar file containing original digitized TIFF images, IMOD files (*com, *log, st, preali, fid, rawtlt,...), and TxBR files (*mat, *txt, preali, rawtlt, fid, ...) of high pressure frozen Hippocampus tissue, showing PSD and vesicles.
Animation description
Aligned tilt series of a synaptic contact in hippocampal area CA1 neuropil imaged using intermediate voltage electron microscopy, from tissue that was prepared by a chemical fixation followed by high pressure freezing.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3650*  Cite 
Project: P1243
Project name
High Pressure Freezing and Freeze Substitution
Description
This project is designed to achieve ultimate ultrastructure of animal tissues.
Funding agency
NIH
Leader(s)
Mark Ellisman
Gina Sosinsky
Ying Jones
Start date
01-01-2004
End date
unspecified
 
Experiment
Experiment ID
3396
Experiment date
08-17-2006
Title
HPF/FS on fixed rat brain and nerve tissues of rat
Purpose
To achieve better ultrastructure of brain and nerve tissue.
Experimenter(s)
Ying Jones
Microscopy product
Microscopy product ID
3650
Instrument
JEOL 4000#1
Microscopy type
IVEM
Product type
DOUBLE TILT
Image basename
A2hippoHPF
Spatial Axis Image Size Pixel Size
X 2242px
Y 3340px
Subject
Species
rat sprague dawley
Scientific name
rat
Strain
sprague dawley
Group by
Type of fixation
Treatment
Chemical fixation followed by high pressure freezing
Age
23 days
Age class
young adult
Tissue section
Anatomical location
hippocampus
Microtome
leica vibratome
Thickness
0.5 µm
Specimen description
Organ
brain
System
central nervous
Structure
synapse
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
30000
Accelerating voltage
400 kV
Imaging product type
Type
Double tilt
X min range
-60 degrees
X max range
60 degrees
X tilt increment
2 degrees
Y min range
-60 degrees
Y max range
60 degrees
Y tilt increment
2 degrees
Description
double-tilt tomographic dataset of high pressure frozen hippocampus tissue