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Reconstruction
Display image description
A slice by slice view from the backprojected volume of Caulobacter crescentus
Full resolution image description
A .tar file containing 2 MRC format backprojected volumes of Caulobacter crescentus. One is the uncropped version of the volume with XY slices flipped with XZ slices. The other is a smaller cropped and flipped version of the volume. Both volumes have been binned by 2 from the original resolution
Downsampled image description
A single MRC file of the volume that has been cropped and flipped after backprojecting.
File format
MRC
Volume_dimension
1172, 2046, 184
Volume scale
0.001, 0.001, 0.0005
Animation description
A .mpg animation file of the slice by slice view of the backprojected volume.

Image 2D
Display image description
The zero degree tilt view of the raw data acquired from an IVEM of a Caulobacter crescentus sample, strain Snc04
Full resolution image description
A .tar file containing all necessary IMOD processing files for a tomographic reconstruction of Caulobacter crescentus. Raw MRC tilt data is included in this
Animation description
A .mpg animation of the aligned tilt series acquired from an IVEM of a Caulobacter crescentus sample, strain Snc04

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3998*  Cite 
Project: P1220
Project name
Caulobacter
Description
Tomography of Caulobacter crescentus
Funding agency
Department of Energy
Leader(s)
Lucy Shapiro
Harley McAdams
Collaborator(s)
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Start date
07-01-2003
End date
unspecified
 
Experiment
Experiment ID
3374
Experiment date
05-12-2004
Title
Tomographic reconstruction of caulobacter
Purpose
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3998
Instrument
JEOL 4000 #1
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
cb012
Spatial Axis Image Size Pixel Size
X 4096px 0.5 nm/pixels
Y 4096px 0.5 nm/pixels
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus (Snc04)
Strain
Snc04
Treatment
None
Age class
N/A
Tissue section
Anatomical location
N/A
Tissue product storage
N/A
Thickness
0.5 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Magification
0
Accelerating voltage
400 keV
Specimen preparation
Protocol used
1) Induced for 1 hour at 30 degrees2) Labeled for 2 hours at 30 degrees (in presence of 1.8 micromolar ReAsH-EDT2)3) Post fixed with _0.01%_ Acrolein and 2.5% of Glut. in 0.01M Sodium Cocadalyte buffer.4) HPF5) FS by using Epoxy resin as fixative: 20% of the complete Araldite/Epon formulation in acetone. Infiltration and embedding in 100% of resin complex.6) 0.5um sections were cut and collected on gold grids. Sections were post stained with 4% of Osmium followed by Sato lead and finally 10nm was applied on both sides of sections.
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-52 degrees
Max range
66 degrees
Tilt increment
2 degrees