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Specimen Preparation Information Microscopy product ID: 4061 Image basename: 112006dddd Protocol Details Protocol ID: 15692 Protocol name: Immunolabeling P1723 Protocol descriptions: Double labeling immunolabeling of alpha synuclein and mGlR5 Preparation steps: Molecular Localization Molecular Localization Staining Chemical Fixation Microtomy Molecular Localization (15740) Molecular Target (15741) Molecule synuclein isoform alpha Molecular class Snca Entrez ID 20617 PDB ID Notes Probe used (15742) Reagent Probe reference ID Controls omitted primary antibody Notes Antibody (15743) Antibody anti alpha synuclein antibody  more details Clonality monoclonal Antibody type IgG Purification method Reaised in animal mouse Antigent source animal Reference Detection method (15748) Molecule Molecular type antibody Entrez ID Chromagen Alexa 488 Notes Back to top Molecular Localization (15749) Molecular Target (15750) Molecule metabotropic glutamate receptor isoform 5 Molecular class GRM5 Entrez ID 108071 PDB ID Notes Probe used (15751) Reagent Probe reference ID Controls omitted primary antibody Notes Antibody (15752) Antibody anti mGluR5 antibody  more details Clonality polyclonal Antibody type IgG Purification method Reaised in animal rabbit Antigent source animal Reference Detection method (15753) Molecule Molecular type antibody Entrez ID Chromagen Rhodamine Red X Notes Back to top Stain (15765) Stain name DAPI in ProLong  more details Temperature Prepared by Diana Price Notes DAPI is dissolved in ProLong Mounting medium and applied at time of coverslipping Back to top Chemical Fixation (15690) Fixative agent 4% paraformaldehyde-0.1M phosphate buffer  more details Time of fixation Vehicle Temperature 37.0 °C Fixative volume Fixation method perfusion Notes Back to top Microtomy (15691) Anatomic location Tissue product storage Orientation coronal Embedding agent Microtome used Thickness 80.0 μm Temperature Notes Vibratome Back to top Close this window

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