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Reconstruction
Display image description
Single slice through a single tilt tomogram of the Node of Ranvier from mouse sciatic nerve prepared by high pressure freezing and freeze substitution of aldehyde fixed material
Full resolution image description
Serial tomographic reconstruction from 3 serial volumes. The resulting volume was mirrored and merged with the original to from a relatively complete node of Ranvier. The downloadable zip file contains the merged volume in Analyze 7.5 format
Volume_dimension
950, 950, 199
Volume scale
0.005, 0.005, 0.008
Animation description
Annotated movie traversing the slices of the serial tomogram and identifying the segmented objects in the surface rendering

Image 2D
Display image description
Zero tilt image through a 0.500 um section of the Node of Ranvier

Segmentation
Display image description
Manual segmentation of axonal and glial components of the Node of Ranvier using Xvoxtrace v 2.9. Different parts of the myelin sheath were segmented as different objects. Objects were surfaced using both Synu and Amira
Segmentation file description
Zip archive containing the trace file node_marge2.trace and the segmented objects in synu (*.synu) and Amira (*.iv) format. Also included are several renderings of the surface model. These segmentations were produced from the mirrored volume file to mimic a complete node. That is, the original reconstruction of a sagittal section through a node was mirrored to produce the appearance of a single complete node.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:49*  Cite 
Project: P1136
Project name
Dynamics of membrane organization at the node of Ranvier
Description
Serial tomogram of a conventionally prepared peripheral nerve Node of Ranvier
Funding agency
NIH NINDS
Leader(s)
Mark Ellisman
Gina Sosinsky
Collaborator(s)
N.A
Start date
08-01-1995
End date
08-01-1995
 
Experiment
Experiment ID
29
Experiment date
09-26-2000
Title
Serial section electron tomography
Purpose
Structure and modeling
Experimenter(s)
Gina Sosinsky
Tom Deerinck
Microscopy product
Microscopy product ID
49
Instrument
JEOL 4000
Microscopy type
IVEM
Product type
single tilt
Image basename
HPF_node
Spatial Axis Image Size Pixel Size
X 800px 50 nm/pixel
Y 1000px 50 nm/pixel
Subject
Species
mouse
Scientific name
mus musculus
Strain
Unspecified
Age
3 days
Age class
neonate
Tissue section
Anatomical location
dorsal root
Microtome
Leica Ultracut UCT
Tissue product storage
Liquid nitrogen
Thickness
0.5 µm
Specimen description
System
peripheral nervous system
Structure
Node of Ranvier
Tissue
peripheral nerve
Cell type
Schwann cell
Imaging parameters
Type
Electron microscopy product
Magification
25000
Accelerating voltage
400 KeV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees