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Brain mosaics

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Specimen Preparation Information Microscopy product ID: 8141 Image basename: enface Specimen Preparation Tissue group type Conventional EM preparation Ext file name Protocol used Chemical fixation with 2% paraformaldehyde and 2.5% glutaraldehyde via vascular perfusion in 0.15M cacodylate buffer (pH 7.4) at 37 degrees C Neuronal sections cut to a thickness of 300-500 nm were taken on 100 mesh copper clamshell grids. The sections on the grid were stained for 30 minutes to 60 minutes in 2% aqueous uranyl acetate then approximately 15 min in lead salts. 20nm fiducial markers were placed on the specimen. Close this window

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