TIRF time-lapse imaging of EB1-GFP (green) and mCherry-tubulin (red). EB1-GFP is seen on microtubule tips that grow to the cortex but does not accumulate on stable microtubule bundles at the equator, ...

TIRF time-lapse imaging of myosin-GFP (left) and mCherry-tubulin (right) in an S2 cell depleted of kinesin-6 (Pav) by RNAi. Myosin-GFP localization to the equator and loss from the poles does not occu...

Time lapse of mCherry-tubulin by wide-field epifluorescence at the coverslip interface (left), mCherry-tubulin by TIRF (middle), and myosin-GFP by TIRF (right). The acquisition of Cherry-tubulin by TI...

TIRF time lapse of mCherry-tubulin (left), myosin-GFP (middle), and overlay (myosin in green and tubulin in red; right) in a cell with a monopolar spindle from metaphase to anaphase. The S2 cells are...

TIRF time lapse of myosin-GFP (left), mCherry-tubulin (middle), and overlay (myosin-GFP in green and mCherry-tubulin in red) in wild-type S2 cells from metaphase to anaphase. This cell has many astral...

TIRF time lapse of myosin-GFP in a cell with a symmetric monopolar spindle from metaphase to anaphase (cell was depleted of Klp61F [kinesin-5] and BubR1 by RNAi). At anaphase, myosin-GFP concentrates ...

Time lapse of myosin GFP (left, imaged by TIRF microscopy) and mCherry tubulin (middle, imaged by epifluorescence; the spindle is close to the coverslip surface in this cell) during anaphase in a Dros...

A comparison of kinesin-6 anaphase dynamics in a cell with a bipolar or monopolar spindle. (left) TIRF time lapse of kinesin-6 (Pav-GFP; green) and mCherry-tubulin (red) in a wild-type S2 cell with a ...

Time lapse of Aurora-B GFP and mCherry microtubules at the metaphase-to-anaphase transition. The laser illumination was set near to but not achieving complete total internal reflection TIRF to allow v...